The study was undertaken to determine the Electronic information seeking behavior of postgraduate Bio-chemistry students at Federal University of Technology Akure. The overall purpose of the study was to establish the level of computer competency, the attitudes towards E-resources, number of hours spent on the internet, to know which E-resources materials is being used, and to determine their awareness towards library E-resources. The study collected empirical data on electronic information seeking behavior of graduate students. Data was gathered from forty seven (47) students out of sixty (60) postgraduate students. Findings revealed that: there is high computer competency among the postgraduate students, it also confirms that high percentage of postgraduate students have positive attitude towards e-resources, 59.57% of the students spends between one (1) to three (3) hours online at a time. The survey revealed that even though the postgraduate students have high computer competency and high value and interest for e-resources, they have not been using them. Based on these findings it recommends that the university library should improve awareness of their e-resources in other to maximise their use.