The purpose of the present study is to assess factors affecting job satisfaction and its association with anticipated turnover the case of health professionals in Arsi Zone. Descriptive and analytical cross sectional research designs were employed in this study. Primary data was collected using questionnaires from 305 respondents working in selected health centres and hospitals. The collected data was analysed using both descriptive statistics and inferential statistics. The results of the data analysis showed that not enough salary, poor relationship with the staff, non-availability of medical equipment and drugs, and poor training opportunity were found significantly associated with job dissatisfaction. Furthermore, job satisfaction was found to be significantly associated with intention to leave their current job (p value 0.000). From this, it is concluded that different factors affect employee job satisfaction and non-satisfied employees have an intention to leave their jobs. Based upon the findings, recommendations like the government may give attention to the salary of health professionals, managers of health facilities work towards creating good relationship among staffs, and health facilities in collaboration with pharmaceutical fund and supply agent have to fulfill the necessary drugs and medical equipment were forwarded.