In the global scenario, the Business Process Outsourcing (BPO) sector is one of the fast growing sectors after the liberalisation, privatisation, and globalisation, to increase job opportunities day by day. The present study examines the relationship between the workplace stress, well-being, and quality of life. It also aims to investigate the differences between day and night shift workers, males and females, and married and unmarried employees working in BPO sector with respect to experienced workplace stress, well-being and quality of life. In the present study, 120 employees were selected from the different business process companies. The analysis revealed that organisational role stress is significantly correlated with sociability. And, quality of work life was significantly correlated with well-being. However, the difference in gender was found on organisational role stress and some of its dimensions, as well as on quality of work life. Succinctly, married and unmarried employees differed on dimensions of well-being. As far as, shift timings are concerned, day shift employees were found to differ significantly from night shift employees on organisational role stress and its few dimensions, as well as on quality of work life. From the findings of the study, it has been understood that the gender differences, marital status difference, and difference in shift timings have made an impact on the variables such as workplace stress, quality of work life, and wellbeing of BPO employees.