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This research aims to investigate the moderation effect of experience of relationship between quality of work life (QWL) and organizational commitment among university teachers. Sample comprised of 300 teachers within age range from 26 to 64 years (M = 45.28, SD = 10.01) from different faculties of Aligarh Muslim University, Aligarh. Sample was divided into two categories, i.e., high experienced teachers (N = 150) and low experienced teachers (N = 150), based on median (Md = 16.5) of total sample. Obtained data were analyzed by correlation and moderation analysis. Findings showed the significant positive relationship of experience, QWL, and organizational commitment with each other among high or low experienced teachers as well as total teacher sample. Furthermore, significant moderation effect of experience on the relationship between QWL and organizational commitment was reported among low experienced teacher group as well as total teacher sample. In case of high experienced teacher group, significant moderation effect of experience was not observed. These findings substantiate the crucial role of senior university teachers’ in determining their QWL and organizational commitment. Implication of research discussed and suggestions for future research proposed.